Endocrine Abstracts

Introduction: After binding its ligand, a conformational change in estrogen receptor alpha (ERα) occurs, leading to subsequent homodimerization of two monomeric ERs. The dimer then binds to specific DNA-elements or to other transcription factors already bound on gene promoters to regulate target gene expression. Protein-fragment complementation assays in general are simple tools to monitor protein–protein interactions and are useful to visualize the subcellular sites...